Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applications

Methodological Advances in the Culture Manipulation and Utilization  - Preface......Page 1
Part 1......Page 15
01_Embryonic Stem Cells: from Blastocyst to in vitro Differentiation......Page 17
02_Mouse Embryonic Stem Cells Basics from a Fertilized Zygote to These Promising Pluripotent Stem Cells......Page 39
03_Effective Derivation and Manipulation of Mouse Embryonic Stem Cells......Page 59
04_Artificial Acellular Feeder Layer: An Advanced Engineered Extracellular Matrix for Stem Cell Culture......Page 75
05_Biomaterials for In Vitro Expansion of Embryonic Stem Cells......Page 89
06_Surface Engineering to Control Embryonic Stem Cell Fate......Page 107
07_Preservation of Embryonic Stem Cells......Page 127
08_Topographically and Chemically Modified Surfaces for Expansion or Differentiation of Embryonic Stem Cells......Page 153
09_Directed Differentation of Human Embryonic Stem Cells in Combination of Biomaterials......Page 173
Part 2......Page 185
10_Non-invasive Imaging of Human Embryonic Stem Cells Derived Endothelial Cells Using Reporter Genes......Page 187
11_Fourier Transform Infrared Microspectroscopy as a Tool for Embryonic Stem Cell Studies......Page 207
Part 3......Page 233
12_Reprogrammed Parthenogenetic ES Cells - New Choice for Regenerative Medicine......Page 235
13_Reprogramming Somatic Cells by Fusion with Embryonic Stem Cells: Present Status and Prospects in Regenerative Medicine......Page 251
14_Use of Transposon-Transposase Systems for Stable Genetic Modification of Embryonic Stem Cells......Page 273
Part 4......Page 289
15_Gene Transduction Approaches in Human Embryonic Stem Cells......Page 291
16_Pluripotent Stem Cells Induced from Testicular Tissue of a Man with Klinefelter Syndrome (47, XXY) by Four Transcription Factors (OCT4, SOX2, KLF4, and C-MYC)......Page 309
17_Application of Magnet-based Nanofection in Embryonic Stem Cell Research......Page 321
18_Chromosome Engineering in Mouse Embryonic Stem Cells: Addition and Elimination of Targeted Chromosomes......Page 341
19_Microarray Analysis of Undifferentiated and Differentiated Human Pluripotent Stem Cells......Page 357
20_Tracking the Structure of Protein Interaction Network via Multiple Genetic Perturbations on Mouse Embryonic Stem Cells — Implementation of the Entropy Maximization Principle......Page 381
Part 5......Page 395
21_Establishment of Embryonic Stem Cells and Generation of Genetically Modified Rats......Page 415
22_Rat Embryonic Stem Cells: Establishment and Their Use for Transgenesis......Page 429
Part 6......Page 443
23_Assessment of Embryotoxicity and Teratogenicity by the Embryonic Stem Cell Test......Page 445
24_Application of Embryonic Stem Cells as a Novel Tool in Drug Screening......Page 461
25_Human Pluripotent Stem Cell-Derived Neuronal Networks:Their Electrical Functionality and Usability for Modelling and Toxicology......Page 491
26_A Novel Strategy for Drug Discovery and Development by Analyzing the Behavior of ES Cells Cultured on TOSHI (Tissue/Organ Sections for Histopathology)–Substrata......Page 505